Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced and labeled by in vitro transcription.

Addition of anti-GFP antibody results in a supershift.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Kd=0.580uM, Positive control

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

Positive control