Cecropin, CecA, Cec, Cecropin A, Cec A1
Gene model reviewed during 5.49
There is only one protein coding transcript and one polypeptide associated with this gene
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CecA1 using the Feature Mapper tool.
Cecropin mRNAs are induced after injection of a bacterial suspension into adult abdomens. They can also be induced by feeding flies bacteria-containing food.
GBrowse - Visual display of RNA-Seq signalsView Dmel\CecA1 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: CecA1 CG1365
Identified as a gene with significant level of mRNA cycling as assessed by expression analysis using high density oligonucleotide arrays with probe generated from adult heads harvested over six time points over the course of a day. Shows alteration in expression in a Clk mutant background.
Ecol\lacZDpt.PR adults are pricked with a sterile needle dipped in culture pellets of various living microorganisms (distinct bacterial strains, fungal spores or hyphae). Pricking results in a low but clearly detectable expression of all antimicrobial genes and these genes are induced above the background level by specific classes of microorganism.
Antibacterial genes are not induced in L.boulardi resistant strains of Drosophila suggesting they cannot be involved directly in the antiparasitoid response.
The κB motifs of Dpt and CecA1 are not functionally equivalent. Mutants carrying no copies of dl and a single copy of Dif retain their full capacity to express the Dpt and CecA1 genes in response to bacterial challenge.
Genes encoding antibacterial peptides are regulated in a manner distinct from that of Drs, encoding the antifungal peptide. The Cecropins require the spz-cact regulatory cassette for expression at the adult stage.
Transcription is not strongly induced by hymenopteran parasitoids.
The Dif gene product is a sequence specific transcription factor which trans-activates the CecA1 gene in cotransfection assays. Transactivation requires a 40bp upstream element including an insect κΒ-like motif. dl can also activate the CecA1 promoter, but to a lesser extent and in a less sequence-specific manner than Dif. The dl product exerts a dominant negative effect on Dif transactivation of CecA1.
The -760bp region of CecA1 confers inducible and tissue-specific expression. A functional and evolutionary relationship between insect immune response factors and mammalian NF-κB exists.
One of four structural genes, CecA1, CecA2, CecB and CecC, for very similar cecropin proteins, bacteriocidal proteins that are induced in adults in response to bacterial infection. All cecropin genes are coordinately induced by injection (or ingestion) of bacteria, although CecB is expressed at a much lower level.