FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ash1, MRG15
FlyBase ID
FBig0000116036
Interaction Network
Interactions Browser links
ash1 network
MRG15 network
Reported Interactions
FBrf0237270-2.AP.MS
Reference
Huang et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ash1 

Ash1

bait

FLAG tag

ash1 
MRG15 

Mrg15

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ash1 
interaction domain
sufficient binding region
aa 1146-2226
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Huang et al., 2017)
FBrf0237270-4.coIP.WB
Reference
Huang et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ash1 

Ash1

bait

FLAG tag

ash1 
ash1 
ash1 
MRG15 

Mrg15

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ash1 
interaction domain
sufficient binding region
aa 1146-1530
ash1 
interaction domain
necessary binding region
aa 1146-1530
ash1 
interaction domain residue 1288
mutation decreasing interaction
aa 1288

R1288A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Huang et al., 2017)
FBrf0237270-6.coIP.WB
Reference
Huang et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ash1 

Ash1

bait
MRG15 

Mrg15

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Huang et al., 2017)
FBrf0237270-7.coIP.WB
Reference
Huang et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
MRG15 

Mrg15

bait
ash1 

Ash1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Huang et al., 2017)
FBrf0238560-1.AF.MW
Reference
Schmähling et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
MRG15 

MRG15

bait

Strep tag

ash1 

Ash1

prey
ash1 
ash1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ash1 
C-terminal domain
sufficient binding region
aa 1041-2226
ash1 
FxLP motif
mutation disrupting interaction
aa 1294,1296

F1294R,L1296R

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Hi-5 cell line; bait produced from baculovirus expression construct; prey produced from baculovirus expression construct.

(Schmähling et al., 2018)
FBrf0238560-5.TAP.MS
Reference
Schmähling et al., 2018
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ash1 

Ash1

bait

TAP tag

MRG15 

MRG15

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Schmähling et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)