FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Rab35, sn
FlyBase ID
FBig0000116046
Interaction Network
Interactions Browser links
Rab35 network
sn network
Reported Interactions
FBrf0208822-1.coIP.WB
Reference
Zhang et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rab35 

Rab35

bait

Myc tag

Rab35 
sn 

Fascin

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rab35 
N-terminal domain residue 22
mutation decreasing interaction
aa 22

S22N, coordinates relative to Rab35-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HeLa cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2009)
FBrf0208822-2.PD.MW
Reference
Zhang et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rab35 

Rab35

bait

GST tag

Rab35 
sn 

Fascin

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rab35 
N-terminal domain residue 22
mutation decreasing interaction
aa 22

S22N, coordinates relative to Rab35-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Zhang et al., 2009)

The Rab35-sn interaction was greater with the GTP bound form of Rab35.

(Zhang et al., 2009)
FBrf0208822-3.coIP.WB
Reference
Zhang et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Rab35 

Rab35

bait
sn 

Fascin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Zhang et al., 2009)

The Rab35-sn interaction was greater with the GTP bound form of Rab35.

(Zhang et al., 2009)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)