Source was cell extract of S2 cell line; bait produced from endogenous gene or produced as a recombinant fusion protein in bacterial system; prey produced from endogenous gene.

The eIF4E1-AGO2 interaction is strongly enhanced by the presence of siRNA and target. The eIF4E1-AGO2 interaction is abolished by treatment with RNase or by mutating RNA target sites complementary to siRNA. The eIF4E1-AGO2 interaction decreases by treatment with increasing amounts of Thor.

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

The eIF4E1-AGO2 interaction is strongly enhanced by the presence of siRNA and target.