FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
sd, E2f1
FlyBase ID
FBig0000116066
Interaction Network
Interactions Browser links
sd network
E2f1 network
Reported Interactions
FBrf0237426-1.coIP.WB
Reference
Zhang et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
sd 

Sd

bait

HA tag

E2f1 

E2F1

prey
E2f1 
E2f1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
E2f1 
DNA binding domain
mutation decreasing interaction
aa 259,260

L259E,G260F, coordinates relative to E2f1-PA

E2f1 
DNA binding domain
mutation disrupting interaction
aa 296

D296A, coordinates relative to E2f1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Zhang et al., 2017)
FBrf0237426-5.coIP.WB
Reference
Zhang et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

Sd

bait

HA tag

sd 
sd 
E2f1 

E2F1

prey
E2f1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sd 
N-terminal domain
sufficient binding region
aa unspecified

N-terminal half

sd 
C-terminal domain
sufficient binding region
aa unspecified

C-terminal half

E2f1 
N-terminal domain
sufficient binding region
aa 1-524

Q525 mutated to stop, coordinates relative to E2f1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2017)

The sd-yki interaction decreases upon coexpression of Rbf.

(Zhang et al., 2017)
FBrf0237426-6.PD.WB
Reference
Zhang et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E2f1 

E2F1

bait

Myc tag

sd 

Sd

prey

GST tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait immunopurified from transfected HEK293T cells; prey produced as a recombinant fusion protein in bacterial system.

(Zhang et al., 2017)

The sd-E2f1 interaction decreases upon coexpression of yki.

(Zhang et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)