FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
polo, stg
FlyBase ID
FBig0000116137
Interaction Network
Interactions Browser links
polo network
stg network
Reported Interactions
FBrf0237276-4.coIP.WB
Reference
Kachaner et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
polo 

polo

bait

GFP tag

polo 
stg 

Cdc25

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
polo 
NLS domain
mutation decreasing interaction
aa 106-118

K106A,K107A,R108A,K115A,R116A,R117A,K118A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kachaner et al., 2017)

The polo-stg interaction is decreased by treatment with a Cdk1 inhibitor suggesting that Cdk1 function enhances the interaction.

(Kachaner et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)