FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
aub, wisp
FlyBase ID
FBig0000116168
Interaction Network
Interactions Browser links
aub network
wisp network
Reported Interactions
FBrf0237109-1.coIP.WB
Reference
Dufourt et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aub 

Aub

bait

GFP tag

wisp 

Wispy

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Dufourt et al., 2017)

The aub-wisp interaction was observed with and without RNase treatment.

(Dufourt et al., 2017)
FBrf0237109-2.coIP.WB
Reference
Dufourt et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
wisp 

Wisp

bait
aub 

Aub

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type or mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Dufourt et al., 2017)

The aub-wisp interaction was observed with RNase treatment in wildtype or csul or tud mutant backgrounds.

(Dufourt et al., 2017)
FBrf0237109-3.PD.WB
Reference
Dufourt et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
wisp 

Wispy

bait

GST tag

wisp 
aub 

Aub

prey

HA tag

aub 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
aub 
N-terminal domain
sufficient binding region
aa 1-482

coordinates relative to aub-PA

wisp 
interaction domain
sufficient binding region
aa 636-746aa 1-713aa 702-1373

coordinates relative to wisp-PA

coordinates relative to wisp-PA

coordinates relative to wisp-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced by in vitro translation.

(Dufourt et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)