Brat
zld
Source was larval brains; bait produced from endogenous gene; prey produced from endogenous gene.
The brat-zld interaction was reported with the zld-RB transcript.
His tag
[32]P label
several putative brat binding sites, (A/U)UGUU(A/G/U), in zld-RB 3-prime UTR, mutations in which abolish interaction.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.
