FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
trpl, Fkbp59
FlyBase ID
FBig0000116407
Interaction Network
Interactions Browser links
trpl network
Fkbp59 network
Reported Interactions
FBrf0139716-11.coIP.WB
Reference
Goel et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
trpl 

TRPL

bait
Fkbp59 

dFKBP59

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Goel et al., 2001)
FBrf0139716-13.CH.WB
Reference
Goel et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trpl 

TRPL

bait

GST tag

trpl 
trpl 
trpl 
Fkbp59 

dFKBP59

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trpl 
interaction domain
sufficient binding region
aa 651-1124
trpl 
interaction domain residue 702
mutation disrupting interaction
aa 702

P702Q

trpl 
interaction domain residue 709
mutation disrupting interaction
aa 709

P709Q

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus and Sf9 cell system; prey produced from transfected construct in Sf9 cell lysate.

(Goel et al., 2001)

Treatment with FK506 decreases the FKBP59-trpl interaction.

(Goel et al., 2001)
FBrf0139716-2.coIP.WB
Reference
Goel et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Fkbp59 

dFKBP59

bait

GST tag

trpl 

TRPL

prey

FLAG tag

trpl 
trpl 
trpl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trpl 
interaction domain
necessary binding region
aa 803-1124
trpl 
interaction domain residue 702
mutation disrupting interaction
aa 702

P702Q

trpl 
interaction domain residue 709
mutation disrupting interaction
aa 709

P709Q

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Goel et al., 2001)

Treatment with FK506 decreases the FKBP59-trpl interaction.

(Goel et al., 2001)
FBrf0139716-3.coIP.WB
Reference
Goel et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trpl 

TRPL

bait

FLAG tag

Fkbp59 

dFKBP59

prey

GST tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Goel et al., 2001)

Treatment with FK506 decreases the FKBP59-trpl interaction.

(Goel et al., 2001)
FBrf0139716-8.coIP.WB
Reference
Goel et al., 2001
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trpl 

TRPL

bait
Fkbp59 

dFKBP59

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Goel et al., 2001)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)