FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Smn, slmb
FlyBase ID
FBig0000116439
Interaction Network
Interactions Browser links
Smn network
slmb network
Reported Interactions
FBrf0237738-1.coIP.WB
Reference
Gray et al., 2018
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Smn 

SMN

bait

FLAG tag

slmb 

Slmb

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Gray et al., 2018)
FBrf0237738-14.coIP.WB
Reference
Gray et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Smn 

SMN

bait

FLAG tag, a version of Smn, vSmn, vertebratized by the following mutations- A193S, A197S and insertion of MGLR after Y208

Smn 
Smn 
slmb 

Slmb

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Smn 
YG Box
mutation increasing interaction

a version of vSmn, vSmnΔ7, designed to mimic human SmnΔ7 where MEMLA is inserted after Y208 and truncated therafter.

Smn 
YG Box residue 201
mutation decreasing interaction
aa 201

S201A in the context of vSmnΔ7.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gray et al., 2018)
FBrf0237738-4.coIP.MS
Reference
Gray et al., 2018
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Smn 

SMN

bait

FLAG tag

slmb 

Slmb

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Gray et al., 2018)
FBrf0237738-9.coIP.WB
Reference
Gray et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
slmb 

Slmb

bait

FLAG tag

Smn 

SMN

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Gray et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)