FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Tis11, Ldh
FlyBase ID
FBig0000116442
Interaction Network
Interactions Browser links
Tis11 network
Ldh network
Reported Interactions
FBrf0238520-1.EMSA.A
Reference
de Toeuf et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tis11 

dTIS11

bait

His tag

Ldh 

ImpL3

prey

[32]P label

Ldh 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ldh 
ARE enriched region of 3-prime UTR
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.

(de Toeuf et al., 2018)
FBrf0238520-2.ITC
Reference
de Toeuf et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tis11 

dTIS11

bait

His tag

Ldh 

ImpL3

prey
Ldh 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ldh 
ARE enriched region of 3-prime UTR
sufficient binding region
uguuuauuuuuaguccaccucucaauuuauuuauuauuuuuauga
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(de Toeuf et al., 2018)

Kd=2.4uM

(de Toeuf et al., 2018)
FBrf0238520-4.coIP.PCR
Reference
de Toeuf et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Tis11 

dTIS11

bait
Ldh 

ImpL3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(de Toeuf et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)