ImpL3, Imp-L3, Ecdysone-inducible gene L3, dLdh, lactic DH
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.51
Gene model reviewed during 5.45
Gene model reviewed during 5.56
1.5 (northern blot)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ldh using the Feature Mapper tool.
Ldh transcripts are abundant during late embryogenesis and in larval stages. Transcripts are present at low levels in pupae and increase again in adults. Ldh transcripts are detected in cultured imaginal discs and levels increase with exposure to 20-hydroxyecdysone. In situ hybridization shows that Ldh transcripts are abundantly expressed in somatic muscles from stage 13 on and are detected as early as stage 11. Staining is also observed in the posterior spiracles, pharyngeal muscles, and proventriculus by stage 15.
Levels of Ldh protein were measured by assaying for LDH activity. LDH activity is detected in cultured imaginal discs and is significantly higher in discs cultured with 20-hydroxyecdysone. LDH activity was found to be low in third instar larval leg discs but high in prepupal leg discs in the tarsal segments and the presumptive tibia. LDH activity localizes to somatic mesoderm and later to somatic muscle cells in embryos. Activity is also detected in the posterior spiracles, pharyngeal muscles, and proventriculus by stage 15.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Ldh in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Ldh ImpL3
Source for merge of: ImpL3 CG10160
The ImpL3 lactate dehydrogenase product may have a similar function in imaginal disc morphogenesis as it does in muscle contraction, and maintains levels of metabolism required for active actin-myosin contraction in an environment characterized by reduced oxygen availability.
Temporal profile of gene expression is altered in Eip74EF mutant background.
Encodes a transcript associated with membrane-bound polysomes in imaginal discs and expressed only in response to 20-hydroxyecdysone (20 HOE). Expression studied both in vitro and in vivo. A 'late' gene involved in the eversion to the exterior of the elongated regions of discs by means of local changes in cell shape (Osterbur, Fristrom, Natzle, Tojo and Fristrom, 1988).