FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Smurf
FlyBase ID
FBig0000116500
Interaction Network
Interactions Browser links
Smurf network
Reported Interactions
FBrf0238147-3.coIP.WB
Reference
Li et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Smurf 

Smurf

bait

FLAG tag, Smurf N-terminus

Smurf 

Smurf

prey

HA tag, Smurf HECT domain

Smurf 
Smurf 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Smurf 
HECT domain
sufficient binding region
aa unspecified
Smurf 
N-terminal domain
sufficient binding region
aa unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Co-expression of Gprk2 inhibits the Smurf-N-terminus-Smurf-HECT domain interaction. Mutation of the Gprk2 phosphotarget residues in the Smurf-N-terminus renders Smurf insensitive to Gprk2 inhibition.

(Li et al., 2018)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Li et al., 2018)
External Crossreferences and Linkouts ( 0 )
References (1)