FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
piwi, Kap-α3
FlyBase ID
FBig0000116727
Interaction Network
Interactions Browser links
piwi network
Kap-α3 network
Reported Interactions
FBrf0239240-1.coIP.WB
Reference
Yashiro et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
OSC
Participants
Corresponds to
Reported as
Role
Note
Kap-α3 

Impα3

bait
piwi 

Piwi

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of OSC cell line; bait produced from endogenous gene; prey produced from transfected construct.

(Yashiro et al., 2018)

Decreasing the amount of Gasz via RNAi reduces the piwi-Kap-α3 interaction.

(Yashiro et al., 2018)
FBrf0239240-2.coIP.WB
Reference
Yashiro et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
OSC
Participants
Corresponds to
Reported as
Role
Note
piwi 

Piwi

bait

Myc tag

piwi 
piwi 
Kap-α3 

Impα3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
piwi 
N-terminal domain, residues 9-12
mutation decreasing interaction
aa 9-12

RGRRRP to RGAAAA

piwi 
N-terminal domain, residues 31,34
mutation decreasing interaction
aa 31,34

K31A, R34A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of OSC cell line; bait produced from endogenous gene; prey produced from transfected construct.

(Yashiro et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)