FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Vap33, l(2)gl
FlyBase ID
FBig0000116746
Interaction Network
Interactions Browser links
Vap33 network
l(2)gl network
Reported Interactions
FBrf0239211-1.PD.MS
Reference
Portela et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
l(2)gl 

Lgl

bait

SBP tag

Vap33 

Vap33

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Portela et al., 2018)

AvgP SAINT value = 0.998

(Portela et al., 2018)
FBrf0239211-2.coIP.WB
Reference
Portela et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Vap33 

Vap33

bait

V5 tag

l(2)gl 

Lgl

prey

SBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Portela et al., 2018)
FBrf0239211-3.PLA
Reference
Portela et al., 2018
Description
physical association
Assay
Collection
Source/Stage
eye disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
l(2)gl 

Lgl

bait

GFP tag

Vap33 

Vap33

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was eye discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Portela et al., 2018)
FBrf0239211-4.PLA
Reference
Portela et al., 2018
Description
physical association
Assay
Collection
Source/Stage
eye disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Vap33 

Vap33

bait

HA tag

l(2)gl 

Lgl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was eye discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Portela et al., 2018)
FBrf0239211-8.PLA
Reference
Portela et al., 2018
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
l(2)gl 

Lgl

bait

GFP tag

Vap33 

Vap33

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was wing discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Portela et al., 2018)
FBrf0239211-9.PLA
Reference
Portela et al., 2018
Description
physical association
Assay
Collection
Source/Stage
salivary gland / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
l(2)gl 

Lgl

bait

GFP tag

Vap33 

Vap33

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was salivary glands of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Portela et al., 2018)
FBrf0258800-5.PLA
Reference
Portela et al., 2024
Description
physical association
Assay
Collection
Source/Stage
eye disc / third instar larval stagebrain / third instar larval stagesalivary gland / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Vap33 

Vap33

neutral component
Vap33 

Vap33

neutral component
Vap33 

Vap33

neutral component
l(2)gl 

Lgl

neutral component

GFP tag

l(2)gl 

Lgl

neutral component

GFP tag

l(2)gl 

Lgl

neutral component

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control

(Portela et al., 2024)

Source was larval eye discs, brains and salivary glands of transgenic fly line; Vap33 produced from endogenous gene; l(2)gl produced from transgenic construct.

(Portela et al., 2024)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)