Orc3, xmas Physical Interaction report

General Information

Interaction Type

physical association

Interacting Genes

Orc3, xmas

FlyBase ID

FBig0000116991

Interaction Network

Interactions Browser links

Orc3 network
xmas network

Reported Interactions

FBrf0232523-10.coIP.WB

Reference

Kopytova et al., 2016

Description

physical association

Assay

anti bait coimmunoprecipitation

western blot

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

xmas

Xmas-2

bait

Orc3

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Embryonic nuclear extract was treated with DNase and RNase.

(Kopytova et al., 2016)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kopytova et al., 2016)

FBrf0232523-25.coIP.WB

Reference

Kopytova et al., 2016

Description

physical association

Assay

anti bait coimmunoprecipitation

western blot

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Orc3

bait

xmas

Xmas-2

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Embryonic nuclear extract was treated with DNase and RNase.

(Kopytova et al., 2016)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kopytova et al., 2016)

FBrf0232523-49.coIP.WB

Reference

Kopytova et al., 2016

Description

physical association

Assay

anti tag coimmunoprecipitation

anti tag western blot

Collection

Source/Stage

Cell line used

S2-DGRC

Participants

Corresponds to

Reported as

Role

Note

xmas

Xmas-2

bait

HA tag

Orc3

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kopytova et al., 2016)

FBrf0232523-56.coIP.WB

Reference

Kopytova et al., 2016

Description

physical association

Assay

anti tag coimmunoprecipitation

anti tag western blot

Collection

Source/Stage

Cell line used

S2-DGRC

Participants

Corresponds to

Reported as

Role

Note

Orc3

bait

FLAG tag

xmas

Xmas2

prey

HA tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kopytova et al., 2016)

FBrf0248376-1.coIP.WB

Reference

Kurshakova et al., 2021

Description

physical association

Assay

anti tag coimmunoprecipitation

western blot

Collection

Source/Stage

Cell line used

S2-unspecified

Participants

Corresponds to

Reported as

Role

Note

xmas

Xmas-2

bait

FLAG tag

xmas

Orc3

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

xmas

C-terminal domain

sufficient binding region

aa 835-1359

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Kurshakova et al., 2021)

FBrf0248376-2.coIP.WB

Reference

Kurshakova et al., 2021

Description

physical association

Assay

anti tag coimmunoprecipitation

anti tag western blot

Collection

Source/Stage

Cell line used

S2-unspecified

Participants

Corresponds to

Reported as

Role

Note

xmas

Xmas-2

bait

FLAG tag

xmas

Orc3

prey

HA tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

xmas

C-terminal domain

sufficient binding region

aa 643-1359

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kurshakova et al., 2021)

FBrf0248376-3.coIP.WB

Reference

Kurshakova et al., 2021

Description

physical association

Assay

anti tag coimmunoprecipitation

anti tag western blot

Collection

Source/Stage

Cell line used

S2-unspecified

Participants

Corresponds to

Reported as

Role

Note

Orc3

bait

HA tag

xmas

Xmas-2

prey

FLAG tag

xmas

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

xmas

C-terminal domain

sufficient binding region

aa 835-1359

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kurshakova et al., 2021)

External Crossreferences and Linkouts ( 0 )

References (2)

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