FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
lola, fru
FlyBase ID
FBig0000117445
Interaction Network
Interactions Browser links
lola network
fru network
Reported Interactions
FBrf0241165-1.coIP.WB
Reference
Sato et al., 2019
Description
physical association
Assay
Collection
Source/Stage
eye-antennal disccentral nervous system / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
lola 

lola

bait

anti-lola29M male specific isoform used for IP

fru 

fru

prey

fruBM male specific isoform detected

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was CNS/eye-antennal disc of transgenic fly line; bait produced from endogenous gene; prey produced from transgenic construct.

(Sato et al., 2019)
FBrf0241165-2.coIP.WB
Reference
Sato et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
lola 

lola

bait

V5 tag, lola29M male specific isoform used

lola 
fru 

fru

prey

FLAG tag, fruBM male specific isoform used

fru 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
fru 
BTB domain
necessary binding region
aa 3-113

coordinates relative to fru-PA

lola 
BTB domain
necessary binding region
aa 1-118

coordinates relative to lola-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Sato et al., 2019)
FBrf0260563-59.Y2H
Reference
Bonchuk et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
lola 

lola

bait

fused to GAL4 DNA-binding domain

lola 
fru 

Fruitless

prey

fused to GAL4 activation domain

fru 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
lola 
TTK-type BTB domain
sufficient binding region
aa 1-120
fru 
TTK-type BTB domain
sufficient binding region
aa 1-118
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bonchuk et al., 2024)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bonchuk et al., 2024)
External Crossreferences and Linkouts ( 0 )
References (2)