FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
ens, Nin
FlyBase ID
FBig0000117608
Interaction Network
Interactions Browser links
ens network
Nin network
Reported Interactions
FBrf0241414-1.Y2H
Reference
Rosen et al., 2019
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ens 

Ens

bait

fused to GAL4 DNA-binding domain

Nin 

Bsg25D

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD, sequence common to all Bsg25D clones recovered corresponds to aa 487-526.

(Rosen et al., 2019)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Rosen et al., 2019)
FBrf0241414-2.coIP.WB
Reference
Rosen et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ens 

Ens

bait

GFP tag

Nin 

Bsg25D

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Rosen et al., 2019)
FBrf0260137-2.coIP.WB
Reference
Tillery et al., 2024
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ens 

ens

bait

GFP tag

Nin 

Nin

prey

RFP-MyC tag

Nin 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nin 
interaction domain
sufficient binding region
aa 568-777

coordinates relative to Nin-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control

(Tillery et al., 2024)

Source was larvae of transgenic fly line; bait and prey produced from tagged transgenic construct.

(Tillery et al., 2024)
FBrf0260137-3.coIP.WB
Reference
Tillery et al., 2024
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nin 

Nin

bait

GFP tag

ens 

ens

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control

(Tillery et al., 2024)

Source was larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Tillery et al., 2024)
External Crossreferences and Linkouts ( 0 )
References (2)