Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).
Modified yeast two-hybrid for membrane proteins. A bait-prey interaction forms a ubiquitin molecule and allows release of the tethered transcription factor.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Modified yeast two-hybrid for membrane proteins. A bait-prey interaction forms a ubiquitin molecule and allows release of the tethered transcription factor.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was larval trachea of wild-type fly line; proteins produced from endogenous gene or tagged transgenic construct.
The PLA signal develops only if probes to the two factors are in close proximity to each other (within tens of nanometers).
