FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Hmr, Nlp
FlyBase ID
FBig0000117675
Interaction Network
Interactions Browser links
Hmr network
Nlp network
Reported Interactions
FBrf0240882-7.coIP.WB
Reference
Anselm et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Nlp 

NLP

bait

V5 tag

Nlp 
Nlp 
Nlp 
Nlp 
Nlp 
Hmr 

HMR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nlp 
interaction domain residue 6
mutation disrupting interaction
aa 6

F6E, coordinates relative to Nlp-PA

Nlp 
interaction domain residue 62
mutation decreasing interaction
aa 62

I62E, coordinates relative to Nlp-PA

Nlp 
interaction domain residue 79
mutation disrupting interaction
aa 79

V79E, coordinates relative to Nlp-PA

Nlp 
tail domain
necessary binding region
unspecified
Nlp 
tail domain residues 112-142
mutation disrupting interaction
aa 112-142

series of D or E to A substitutions, coordinates relative to Nlp-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Anselm et al., 2018)
FBrf0250942-15.coIP.MS
Reference
Lukacs et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Hmr 

HMR

bait

Flag tag

Hmr 
Nlp 

NLP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Hmr 
MADF and unstructured domain residues 371 and 527
mutation disrupting interaction
aa 371;527

coordinates relative to Hmr-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Over-expression of wild type Hmr and Myc-tagged Lhr results in decreased interaction.

(Lukacs et al., 2021)

Source was nuclear extracts of an S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lukacs et al., 2021)
FBrf0250942-25.coIP.MS
Reference
Lukacs et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Nlp 

NLP

bait
Hmr 

HMR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extracts of an S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lukacs et al., 2021)
FBrf0250942-3.coIP.MS
Reference
Lukacs et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Hmr 

HMR

bait
Nlp 

NLP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extracts of an S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lukacs et al., 2021)
External Crossreferences and Linkouts ( 0 )
References (2)