Usp7, yki Physical Interaction report

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

yki

Yki

bait

Myc tag

yki

Usp7

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

yki

N-terminal domain

sufficient binding region

aa 1-250

coordinates relative to yki-PH

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Sun et al., 2019)

FBrf0241572-2.coIP.WB

Reference

Sun et al., 2019

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Usp7

bait

FLAG tag

Usp7

yki

Yki

prey

Myc tag

yki

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

yki

N-terminal domain

sufficient binding region

aa 1-250

coordinates relative to yki-PH

Usp7

C-terminal domain

sufficient binding region

aa 587-1129

coordinates relative to Usp7-PB

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Sun et al., 2019)

FBrf0241572-3.coIP.WB

Reference

Sun et al., 2019

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

yki

Yki

bait

Usp7

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Sun et al., 2019)

FBrf0241572-4.PD.WB

Reference

Sun et al., 2019

Description

physical association

Assay

pull down

western blot

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Usp7

bait

GST tag

yki

Yki

prey

Myc tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

(Sun et al., 2019)

FBrf0241572-6.PD.WB

Reference

Sun et al., 2019

Description

physical association

Assay

pull down

western blot

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

yki

Yki

bait

GST tag

yki

Usp7

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

yki

N-terminal domain

sufficient binding region

aa 1-250

coordinates relative to yki-PH

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

(Sun et al., 2019)

FBrf0260347-11.coIP.WB

Reference

Sun et al., 2024

Description

physical association

Assay