FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
sip2, feo
FlyBase ID
FBig0000119665
Interaction Network
Interactions Browser links
sip2 network
feo network
Reported Interactions
FBrf0241925-1.Y2H
Reference
Swider et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
feo 

Feo

bait

fused to GAL4 DNA-binding domain

sip2 

FIP

prey

fused to GAL4 activation domain

sip2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sip2 
C-terminal domain
sufficient binding region
aa 220-657

coordinates relative to sip2-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Swider et al., 2019)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Swider et al., 2019)
FBrf0241925-2.Y2H
Reference
Swider et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sip2 

FIP

bait

fused to GAL4 DNA-binding domain

sip2 
feo 

Feo

prey

fused to GAL4 activation domain

feo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sip2 
C-terminal domain
sufficient binding region
aa 439-657

coordinates relative to sip2-PB

feo 
N-terminal domain
sufficient binding region
aa 1-346

coordinates relative to feo-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Swider et al., 2019)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Swider et al., 2019)
FBrf0241925-3.coIP.WB
Reference
Swider et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
feo 

Feo

bait

GFP tag

sip2 

FIP

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Swider et al., 2019)
External Crossreferences and Linkouts ( 0 )
References (1)