Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two-hybrid system: yeast LexA-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

PDZ-GEF binds to the wild-type form of Rap1 as well as to both a constitutively active (CA) and a dominant-negative (DN) mutant form of Rap1.

Two-hybrid system: yeast LexA-BD/GAl4-AD