FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
nxf2, Nxt1
FlyBase ID
FBig0000119854
Interaction Network
Interactions Browser links
nxf2 network
Nxt1 network
Reported Interactions
FBrf0243113-4.coIP.MS
Reference
Fabry et al., 2019
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nxf2 

Nxf2

bait

GFP tag

Nxt1 

Nxt1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Fabry et al., 2019)
FBrf0243113-6.coIP.WB
Reference
Fabry et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
nxf2 

Nxf2

bait

FLAG tag

nxf2 
nxf2 
Nxt1 

Nxt1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nxf2 
NPC-binding domain
necessary binding region
unspecified
nxf2 
NTF2 domain residues 754-756
mutation disrupting interaction
aa 754-756

ERL to AGA, coordinates relative to nxf2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Fabry et al., 2019)
FBrf0243126-17.coIP.MS
Reference
ElMaghraby et al., 2019
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nxt1 

Nxt1

bait

LAP tag or tdTomato-HA tag

nxf2 

Nxf2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(ElMaghraby et al., 2019)
FBrf0243162-14.XL
Reference
Batki et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nxt1 

Nxt1

neutral component
Nxt1 

Nxt1

neutral component
Nxt1 

Nxt1

neutral component
nxf2 

Nxf2

neutral component
nxf2 

Nxf2

neutral component
nxf2 

Nxf2

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in baculovirus and insect cell system.

(Batki et al., 2019)
FBrf0243162-4.coIP.MS
Reference
Batki et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
OSC
Participants
Corresponds to
Reported as
Role
Note
nxf2 

Nxf2

bait

FLAG tag

Nxt1 

Nxt1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of OSC cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Batki et al., 2019)
FBrf0243162-9.CH.MW
Reference
Batki et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nxf2 

Nxf2

neutral component
nxf2 

Nxf2

neutral component
nxf2 

Nxf2

neutral component
Nxt1 

Nxt1

neutral component
Nxt1 

Nxt1

neutral component
Nxt1 

Nxt1

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in a baculovirus and insect cell system.

(Batki et al., 2019)

Panx, nxf2 and Nxt1 form a trimeric complex, captured as a series of three binary interactions.

(Batki et al., 2019)
FBrf0243284-14.coIP.MS
Reference
Murano et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
OSC
Participants
Corresponds to
Reported as
Role
Note
nxf2 

Nxf2

bait

FLAG tag

Nxt1 

p15

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of OSC cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Murano et al., 2019)
FBrf0243284-16.coIP.WB
Reference
Murano et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
OSC
Participants
Corresponds to
Reported as
Role
Note
Nxt1 

p15

bait

FLAG tag

nxf2 

Nxf2

prey

Myc tag

nxf2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nxf2 
NTF2-like domain
necessary binding region
aa 573-760

coordinates relative to nxf2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of OSC cell line; bait produced from transfected construct; prey produced from transfected construct or endogenous gene.

(Murano et al., 2019)
FBrf0243284-18.coIP.WB
Reference
Murano et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
OSC
Participants
Corresponds to
Reported as
Role
Note
nxf2 

Nxf2

bait

Myc tag

Nxt1 

p15

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of OSC cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Murano et al., 2019)
FBrf0244040-13.coIP.WB
Reference
Zhao et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
OSC
Participants
Corresponds to
Reported as
Role
Note
Nxt1 

dNxt1

bait

GFP tag

nxf2 

dNxf2

prey

Halo tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of OSC cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhao et al., 2019)
FBrf0244040-29.CL.MS
Reference
Zhao et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nxf2 

dNxf2

neutral component
nxf2 
nxf2 

dNxf2

neutral component
nxf2 
nxf2 

dNxf2

neutral component
Nxt1 

dNxt1

neutral component
Nxt1 

dNxt1

neutral component
Nxt1 

dNxt1

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nxf2 
NTF2 domain
sufficient binding region
aa 555-772

coordinates relative to nxf2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

(Zhao et al., 2019)
FBrf0244040-31.CH.MW
Reference
Zhao et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nxf2 

dNxf2

neutral component
nxf2 

dNxf2

neutral component
nxf2 
nxf2 

dNxf2

neutral component
nxf2 
Nxt1 

dNxt1

neutral component
Nxt1 

dNxt1

neutral component
Nxt1 

dNxt1

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nxf2 
NTF2 and UBA domains
sufficient binding region
aa 569-841

coordinates relative to nxf2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

(Zhao et al., 2019)
FBrf0244040-6.Y2H
Reference
Zhao et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nxf2 

dNxf2

bait

fused to lexA DNA-binding domain

nxf2 
Nxt1 

dNxt1

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nxf2 
NTF2 domain
sufficient binding region
aa 555-787

coordinates relative to nxf2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast lexA-BD/GAL4-AD. The presence of the nxf2 UBA domain decreased the nxf2-Nxt1 interaction.

(Zhao et al., 2019)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Zhao et al., 2019)
FBrf0244040-9.Y3H
Reference
Zhao et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nxf2 

dNxf2

bait

fused to lexA DNA-binding domain

Nxt1 

dNxt1

ancillary
Nxt1 

dNxt1

ancillary
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Three-hybrid system: yeast lexA-BD/GAL4-AD, Nxt1 was coexpressed as a bridging factor in a yeast three-hybrid assay with nxf2 as bait and Panx as prey.

(Zhao et al., 2019)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Zhao et al., 2019)
External Crossreferences and Linkouts ( 0 )
References (5)