FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Nup214, sbr
FlyBase ID
FBig0000119931
Interaction Network
Interactions Browser links
Nup214 network
sbr network
Reported Interactions
FBrf0194866-2.coIP.WB
Reference
Kozlova et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
sbr 

NXF1

bait

GFP tag

sbr 
Nup214 

Nup214

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sbr 
NPC-binding domain
sufficient binding region
aa 371-672

coordinates relative to sbr-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogeous gene.

(Kozlova et al., 2006)
FBrf0244040-16.Y2H
Reference
Zhao et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sbr 

dNxf1

bait

fused to lexA DNA-binding domain

sbr 
Nup214 

Nup214

prey

fused to GAL4 activation domain

Nup214 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nup214 
C-terminal domain
sufficient binding region
aa 853-1711

coordinates relative to Nup214-PA

sbr 
UBA domain
sufficient binding region
aa 601-672

coordinates relative to sbr-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Zhao et al., 2019)

Two-hybrid system: yeast lexA-BD/GAL4-AD.

(Zhao et al., 2019)
FBrf0244040-17.PD.MW
Reference
Zhao et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nup214 

Nup214

bait

GST tag

Nup214 
sbr 

dNxf1

prey
sbr 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nup214 
FG domain
sufficient binding region
aa 1445-1516

coordinates relative to Nup214-PA

sbr 
NTF2 and UBA domain
sufficient binding region
aa 359-672

coordinates relative to sbr-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Zhao et al., 2019)

The Nup214-sbr interaction may not be direct as Nxt1 was also added to the pull-down assay.

(Zhao et al., 2019)
External Crossreferences and Linkouts ( 0 )
References (2)