FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Gyf, Patr-1
FlyBase ID
FBig0000119987
Interaction Network
Interactions Browser links
Gyf network
Patr-1 network
Reported Interactions
FBrf0243046-3.coIP.WB
Reference
Ruscica et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Gyf 

GIGYF

bait

GFP tag

Gyf 
Gyf 
Patr-1 

HPat

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Gyf 
N-terminal domain
sufficient binding region
aa 1-640

coordinates relative to Gyf-PG

Gyf 
GYF domain
mutation disrupting interaction
aa 571,582,590,596

Y571A,F582A,W590A,F596A, coordinates relative to Gyf-PG

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Ruscica et al., 2019)
FBrf0243046-5.coIP.WB
Reference
Ruscica et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Patr-1 

HPat

bait

GFP tag

Patr-1 
Gyf 

GIGYF

prey

HA tag

Gyf 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Gyf 
N-terminal domain
sufficient binding region
aa 1-640

coordinates relative to Gyf-PG

Patr-1 
PPGF domain
mutation decreasing interaction
aa 286,287,289,328,329,331

P286A,P287A,F289A,P328A,P329A,F331A, coordinates relative to Patr-1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ruscica et al., 2019)
FBrf0243046-8.PD.MW
Reference
Ruscica et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gyf 

GIGYF

bait

GST tag

Patr-1 

HPat

prey

MBP tag

Patr-1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Patr-1 
P-rich domain
sufficient binding region
aa 57-499

coordinates relative to Patr-1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Ruscica et al., 2019)
External Crossreferences and Linkouts ( 0 )
References (1)