FB2025_05 , released December 11, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mir-263b, Bx
FlyBase ID
FBig0000119994
Interaction Network
Interactions Browser links
mir-263b network
Bx network
Reported Interactions
FBrf0243268-1.miR-mRNA.LUC
Reference
Nian et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
mir-263b 

miR-263b

bait
Bx 

Bx

prey
Bx 
Bx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Bx 
3-prime UTR
sufficient binding region
Bx 
mir-263b target site
mutation disrupting interaction
GTGCCAA

GTGCCAA to CTACTCG

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

(Nian et al., 2019)

Luciferase reporter assay.

(Nian et al., 2019)
FBrf0243268-2.miR-mRNA.GFP
Reference
Nian et al., 2019
Description
physical association
Assay
Collection
Source/Stage
brain / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-263b 

miR-263b

bait
Bx 

Bx

prey
Bx 
Bx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Bx 
3-prime UTR
sufficient binding region
Bx 
mir-263b target site
mutation disrupting interaction
GTGCCAA

GTGCCAA to CTACTCG

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

GFP reporter assay.

(Nian et al., 2019)

Source was adult brains of transgenic fly line; bait produced from endogenous; prey produced from transgenic reporter construct.

(Nian et al., 2019)
External Crossreferences and Linkouts ( 0 )
References (1)