FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
smg, Not1
FlyBase ID
FBig0000120013
Interaction Network
Interactions Browser links
smg network
Not1 network
Reported Interactions
FBrf0183829-1.TAP.MS
Reference
Semotok et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
smg 

SMG

bait

TAP tag

Not1 

CG1884

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Semotok et al., 2005)
FBrf0256453-4.Y2H
Reference
Pekovic et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
smg 

Smaug

bait

fused to C-ubiquitin

Not1 

Not3

prey

fused to N-ubiquitin

Not1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Not1 
interaction domain residues 752-910
sufficient binding region
aa 752-910

coordinates relative to Not3-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Pekovic et al., 2023)

Two-hybrid system: yeast split ubiquitin

(Pekovic et al., 2023)
External Crossreferences and Linkouts ( 0 )
References (2)