FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Mkrn1, osk
FlyBase ID
FBig0000120238
Interaction Network
Interactions Browser links
Mkrn1 network
osk network
Reported Interactions
FBrf0244799-14.coIP.PCR
Reference
Dold et al., 2020
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mkrn1 

Mkrn1

bait

FLAG tag

Mkrn1 
osk 

osk

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mkrn1 
ZnF1 domain
mutation decreasing interaction
aa 26-33

deletion of ZnF1 domain

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Dold et al., 2020)
FBrf0244799-15.ICLIP.SEQ
Reference
Dold et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Mkrn1 

Mkrn1

bait

FLAG tag

osk 

osk

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Dold et al., 2020)
FBrf0244799-16.coIP.PCR
Reference
Dold et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Mkrn1 

Mkrn1

bait

FLAG tag

Mkrn1 
Mkrn1 
osk 

osk

prey
osk 
osk 
osk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
osk 
3-prime UTR
sufficient binding region
osk 
3-prime UTR Mkrn1 binding site
mutation decreasing interaction
nt 955-978
osk 
3-prime UTR A rich region
mutation decreasing interaction
nt 987-1019
Mkrn1 
ZnF1 domain
mutation decreasing interaction
aa 26-33

deletion of ZnF1 domain, coordinates relative to Mkrn1-PA

Mkrn1 
PAM2 domain residues 90,92
mutation decreasing interaction
aa 90,92

F90A,P92A, coordinates relative to Mkrn1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Dold et al., 2020)

The Mkrn1-osk interaction is decreased with decreased levels of pAbp expression.

(Dold et al., 2020)
External Crossreferences and Linkouts ( 0 )
References (1)