Imp
GFP tag
Mkrn1
FLAG tag
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.
Mkrn1 with a RING domain mutation to increase protein stability was used in interaction assays.
Myc tag
Both wildtype Mkrn1 and Mkrn1 with a RING domain mutation to increase protein stability were used in interaction assays.
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.