FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
wde, Sumo
FlyBase ID
FBig0000120311
Interaction Network
Interactions Browser links
wde network
Sumo network
Reported Interactions
FBrf0244794-10.coIP.WB
Reference
Ninova et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
wde 

wde

bait

GFP tag

Sumo 

SUMO

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ninova et al., 2020)
FBrf0244794-12.coIP.WB
Reference
Ninova et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
wde 

Wde

bait

GFP tag

wde 
wde 
wde 
Sumo 

SUMO

prey

GST tag

Sumo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
wde 
SIM3-6 domains
sufficient binding region
aa 385-655
wde 
SIM7 domain
sufficient binding region
aa 1058-1310
wde 
SIM7 domain residues 1202,1203
mutation disrupting interaction
aa 1202,1203

D1202A,L1203A, coordinates relative to wde-PA

Sumo 
interaction domain residues 26,27,29
mutation disrupting interaction
aa 26,27,29

Q26A,F27A,I29A, coordinates relative to smt3-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from transfected S2 cell extract; prey produced as a recombinant fusion protein in bacterial system.

(Ninova et al., 2020)
External Crossreferences and Linkouts ( 0 )
References (1)