FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
pum, Not1
FlyBase ID
FBig0000120340
Interaction Network
Interactions Browser links
pum network
Not1 network
Reported Interactions
FBrf0244924-1.coIP.WB
Reference
Arvola et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ThermoFischer
Participants
Corresponds to
Reported as
Role
Note
pum 

Pum

bait

FLAG tag

pum 
pum 
Not1 

Not1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pum 
N-terminal domain
sufficient binding region
aa 1-1090

coordinates relative to pum-PA

pum 
N-terminal domain
necessary binding region
aa 1-1090

coordinates relative to pum-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Arvola et al., 2020)
FBrf0254621-1.Y2H
Reference
Haugen et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pum 

Pumillo

bait

fused to GAL4 DNA-binding domain

pum 
pum 
pum 
Not1 

Not1

prey

fused to GAL4 activation domain

Not1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pum 
RD3 domain
sufficient binding region
aa 847-1091
pum 
RD3 domain residue 1033
mutation disrupting interaction
aa 1033

F1033A

pum 
RD3 domain residue 1040
mutation disrupting interaction
aa 1040

F1040A

Not1 
PBR:N1 domain
sufficient binding region
aa 908-995
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Haugen et al., 2022)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Haugen et al., 2022)
FBrf0254621-4.coIP.WB
Reference
Haugen et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Not1 

Not1

bait

Flag tag

pum 

pum

prey

V5 tag

pum 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pum 
RD3 domain
mutation decreasing interaction
aa 847-1091
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of DL1 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Haugen et al., 2022)
External Crossreferences and Linkouts ( 0 )
References (2)