FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
chic, Atg9
FlyBase ID
FBig0000120364
Interaction Network
Interactions Browser links
chic network
Atg9 network
Reported Interactions
FBrf0245495-1.Y2H
Reference
Kiss et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
chic 

profilin

bait

fused to GAL4 DNA-binding domain

chic 
Atg9 

Atg9

prey

fused to GAL4 activation domain

Atg9 
Atg9 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Atg9 
CTD4 domain
sufficient binding region
aa 530-845

coordinates relative to Atg9-PA

Atg9 
putative profilin binding domain
mutation decreasing interaction
aa 809-816

PPRPPAAP to AARAAAAA, coordinates relative to Atg9-PA

chic 
poly-proline binding domain
mutation disrupting interaction
aa 3

W3A, coordinates relative to chic-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Kiss et al., 2020)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Kiss et al., 2020)
FBrf0245495-4.PD.WB
Reference
Kiss et al., 2020
Description
physical association
Assay
Collection
Source/Stage
organism / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
chic 

profilin

bait

GST tag

Atg9 

Atg9

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic adult female extract.

(Kiss et al., 2020)
FBrf0245495-5.coIP.WB
Reference
Kiss et al., 2020
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
chic 

profilin

bait

FLAG tag

Atg9 

Atg9

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Kiss et al., 2020)
External Crossreferences and Linkouts ( 0 )
References (1)