FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mir-8, Vha100-2
FlyBase ID
FBig0000120425
Interaction Network
Interactions Browser links
mir-8 network
Vha100-2 network
Reported Interactions
FBrf0245520-14.miR-mRNA.LUC
Reference
Lim et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
mir-8 

miR-8

bait
mir-8 
Vha100-2 

Vha100-2

prey
Vha100-2 
Vha100-2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Vha100-2 
3-prime UTR
sufficient binding region
Vha100-2 
mir-8 target site
mutation decreasing interaction
UUUUUUGGAUAUUGUCAGUAUUG to UUUUUUGGAUAUUGUCAUAUGUG
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

(Lim et al., 2020)

Luciferase reporter assay.

(Lim et al., 2020)
FBrf0245520-19.miR-mRNA.PCR
Reference
Lim et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
mir-8 

miR-8

bait
Vha100-2 

Vha100-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Lim et al., 2020)

Overexpression of mir-8 reduces Vha100-2 mRNA levels.

(Lim et al., 2020)
External Crossreferences and Linkouts ( 0 )
References (1)