Source was live S2R+ cells; bait produced from transfected construct; prey produced from transfected construct secreted and collected in media.

Bait localizes to cell surface. Prey secreted from transfected cells, collected in media and added to bait expressing cells. Binding of prey to surface of bait cells taken as evidence of direct interaction.

Source was live S2R+ cells; proteins produced from transfected constructs.

Physical interaction of proteins expressed on the surface of cells is inferred from the aggregation of live cells.