FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Eb1, Klp10A
FlyBase ID
FBig0000120709
Interaction Network
Interactions Browser links
Eb1 network
Klp10A network
Reported Interactions
FBrf0184181-1.PD.WB
Reference
Mennella et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Eb1 

Eb1

bait

GST tag

Klp10A 

Klp10A

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract

(Mennella et al., 2005)
FBrf0184181-4.PD.MW
Reference
Mennella et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Eb1 

Eb1

bait

GST tag

Eb1 
Klp10A 

Klp10A

prey

MBP tag

Klp10A 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Eb1 
interaction domain
sufficient binding region
aa 209-279
Klp10A 
N-terminal domain
sufficient binding region
aa 1-229
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Eb1 C-terminus directly binds to the N terminus of Klp10A. Binding of Klp10A to Eb1 is reduced with the addition of Hsap\APC C terminus, previously shown to bind to Eb1.

(Mennella et al., 2005)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system

(Mennella et al., 2005)
External Crossreferences and Linkouts ( 0 )
References (1)