FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
dre4, cal1
FlyBase ID
FBig0000120759
Interaction Network
Interactions Browser links
dre4 network
cal1 network
Reported Interactions
FBrf0228941-2.coIP.MS
Reference
Chen et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
cal1 

CAL1

bait

FLAG tag

dre4 

Dre4

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Chen et al., 2015)
FBrf0228941-6.coIP.WB
Reference
Chen et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
cal1 

CAL1

bait
dre4 

Dre4

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Chen et al., 2015)
FBrf0228941-8.PD.A
Reference
Chen et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dre4 

Dre4

bait

His tag

cal1 

CAL1

prey

[35]S label

cal1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cal1 
interaction domain
sufficient binding region
aa 41-979

coordinates relative to cal1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Chen et al., 2015)
External Crossreferences and Linkouts ( 0 )
References (1)