Various combinations of flag-Mirr and HA-Mirr were mixed with [32]P labeled ACAnnTGT probe. DNA-protein complexes observed by EMSA were used to deduce homodimerization of Mirr.
Addition of N-terminal deletion Mirr protein results in loss of interaction.
Interaction in vitro; proteins were synthesized in vitro by using the coupled transcription/translation rabbit reticulocyte lysate syste