FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Atg6, Rab18
FlyBase ID
FBig0000120921
Interaction Network
Interactions Browser links
Atg6 network
Rab18 network
Reported Interactions
FBrf0247573-1.Y2H
Reference
Takáts et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atg6 

Atg6

bait

fused to GAL4 DNA-binding domain

Rab18 

Rab18

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Takáts et al., 2021)

Atg6 interacted with the A64L, GTP-locked form of Rab18.

(Takáts et al., 2021)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Takáts et al., 2021)
FBrf0247573-2.PD.WB
Reference
Takáts et al., 2021
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rab18 

Rab18

bait

GST tag

Atg6 

Atg6

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic fly extract.

(Takáts et al., 2021)

Atg6 interacted with the A64L, GTP-locked form of Rab18.

(Takáts et al., 2021)
External Crossreferences and Linkouts ( 0 )
References (1)