Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from universal cDNA expression library).
Two-hybrid system
Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus and Sf9 cell system; prey derived from wild-type adult head extract.
Kd = 6nM
Interaction in vitro; proteins produced as recombinant fusion proteins in baculovirus and Sf9 cell system.