FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
ecd, SmD3
FlyBase ID
FBig0000120965
Interaction Network
Interactions Browser links
ecd network
SmD3 network
Reported Interactions
FBrf0248189-10.coIP.WB
Reference
Erkelenz et al., 2021
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
SmD3 

SmD3

bait

HA tag

ecd 

Ecd

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Overexpression of Prp8 increases the SmD3-ecd interaction.

(Erkelenz et al., 2021)

Source was wing discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Erkelenz et al., 2021)
FBrf0248189-3.coIP.WB
Reference
Erkelenz et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
SmD3 

SmD3

bait

FLAG tag

ecd 

Ecd

prey

Myc tag

ecd 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ecd 
C-terminal domain
mutation decreasing interaction
aa 650-684

deletion of the C-terminal 35 aa, coordinates relative to ecd-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Erkelenz et al., 2021)
FBrf0248189-4.coIP.WB
Reference
Erkelenz et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ecd 

Ecd

bait

Myc tag

SmD3 

SmD3

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Erkelenz et al., 2021)
FBrf0248189-5.PD.WB
Reference
Erkelenz et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ecd 

Ecd

bait

His tag

SmD3 

SmD3

prey

His, HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Erkelenz et al., 2021)
FBrf0248189-6.coIP.MS
Reference
Erkelenz et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ecd 

Ecd

bait

Myc tag

ecd 
SmD3 

SmD3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ecd 
C-terminal domain
mutation decreasing interaction
aa 650-684

deletion of the C-terminal 35 aa, coordinates relative to ecd-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Erkelenz et al., 2021)
External Crossreferences and Linkouts ( 0 )
References (1)