Gorab behaves as a dimer in solution.
Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.
The most protected region of Gorab in this assay, termed the dimerization core, is aa 278-284.
Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.
Source was S2 cell line; bait produced from tagged transfected construct; prey produced from transfected construct.
Bait protein was fused to an outer mitochondrial membrane anchor, which targets the chimera to mitochondria. The ability of this chimera to recruit prey protein to mitochondria was taken as evidence of direct physical interaction.
