FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
not, mod(mdg4)
FlyBase ID
FBig0000121050
Interaction Network
Interactions Browser links
not network
mod(mdg4) network
Reported Interactions
FBrf0248278-13.coIP.MS
Reference
Erez et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DRSC
Participants
Corresponds to
Reported as
Role
Note
not 

Not

bait

FLAG,HA tag

mod(mdg4) 

mod(mdg4)

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Erez et al., 2021)
FBrf0248278-2.coIP.WB
Reference
Erez et al., 2021
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
not 

Nonstop

bait
mod(mdg4) 

Mdg4

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Erez et al., 2021)
FBrf0248278-9.TCA
Reference
Erez et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
not 

Not

bait

fused to GAL4 DNA-binding domain

mod(mdg4) 

Mod(mdg4)

prey

fused to GAL4 activation domain

mod(mdg4) 
mod(mdg4) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mod(mdg4) 
interaction domain
sufficient binding region
aa 1-273

coordinates relative to mod(mdg4)-PT

mod(mdg4) 
interaction domain
necessary binding region
aa 118-144

coordinates relative to mod(mdg4)-PT

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Four-hybrid system: yeast GAL4-BD/GAL4-AD, Sgf11 and e(y)2 were coexpressed as bridging factors in a yeast four-hybrid assay with not as bait and mod(mdg4) as prey.

(Erez et al., 2021)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Erez et al., 2021)
External Crossreferences and Linkouts ( 0 )
References (1)