Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
S453A, S455A coordinates relative to Smurf-PA
Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.
interaction is stronger in the presence of constitutively active TKV; Smurf C1029A was used to prevent Mad degradation
Smurf had stronger binding to endogenous phospho-MAD, which is further enhanced by proteasome inhibitor MG-132
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.
Source was cell extract of S2 cell line; bait and prey produced from transfected constructs.
An enzymatically inactive Smurf construct was used.
Positive control
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
