FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Pi3K21B, 18w
FlyBase ID
FBig0000121176
Interaction Network
Interactions Browser links
Pi3K21B network
18w network
Reported Interactions
FBrf0249139-5.coIP.WB
Reference
Tamada et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Pi3K21B 

PI3K-reg

bait

Venus tag

18w 

Toll-2

prey

HA or V5 tag

18w 
18w 
18w 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
18w 
C-terminal domain
necessary binding region
aa 1239-1385

coordinates relative to 18w-PA

18w 
Tyr cluster 2 domain
mutation decreasing interaction
aa 1265,1272,1275

Y1265F,Y1272F,Y1275F, coordinates relative to 18w-PA

18w 
Tyr cluster 1 domain
mutation decreasing interaction
aa 1191,1218,1219,1228

Y1191F,Y1218F,Y1219F,Y1228F, coordinates relative to 18w-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Treatment of cells with Tyr kinase inhibitor, Bosutinib, disrupted the 18w-Pi3K21B interaction.

(Tamada et al., 2021)

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tamada et al., 2021)
FBrf0249139-8.coIP.WB
Reference
Tamada et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
18w 

Toll-2

bait

V5 tag

Pi3K21B 

PI3K-reg

prey

HA tag

Pi3K21B 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Pi3K21B 
SH2 domains
necessary binding region
aa 20-120,325-406

coordinates relative to Pi3K21B-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tamada et al., 2021)
External Crossreferences and Linkouts ( 0 )
References (1)