FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Bfc, srp
FlyBase ID
FBig0000121455
Interaction Network
Interactions Browser links
Bfc network
srp network
Reported Interactions
FBrf0252169-1.Y2H
Reference
Zheng et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Bfc 

Bfc

bait

fused to GAL4 DNA-binding domain

srp 

Srp

prey

fused to GAL4 activation domain

srp 
srp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
srp 
ZnF GATA domain residues 600-850
necessary binding region
aa 600-850
srp 
ZnF GATA domain residues 729-830
mutation decreasing interaction
aa 729-830
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Zheng et al., 2021)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Zheng et al., 2021)
FBrf0252169-2.coIP.WB
Reference
Zheng et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Bfc 

Bfc

bait

FLAG tag

srp 

Srp

prey

HA tag

srp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
srp 
ZnF GATA domain residues 600-850
necessary binding region
aa 600-850
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zheng et al., 2021)
FBrf0252169-3.EMSA.WB
Reference
Zheng et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
srp 

Srp

neutral component

GST tag

srp 

Srp

neutral component

GST tag

srp 

Srp

neutral component

GST tag

Bfc 

Bfc

neutral component

His tag

Bfc 

Bfc

neutral component

His tag

Bfc 

Bfc

neutral component

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

srp and Bfc were mixed with biotinylated DNA. DNA-protein complexes observed by EMSA were used to deduce protein-protein interactions.

(Zheng et al., 2021)

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Zheng et al., 2021)
External Crossreferences and Linkouts ( 0 )
References (1)