FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
polo, bsd
FlyBase ID
FBig0000121520
Interaction Network
Interactions Browser links
polo network
bsd network
Reported Interactions
FBrf0252671-1.PD.MS
Reference
Yang et al., 2022
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
bsd 

Bsd

bait

GST tag

polo 

Polo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Yang et al., 2022)
FBrf0252671-2.coIP.WB
Reference
Yang et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
polo 

Polo

bait

Flag tag

bsd 

Bsd

prey

Myc tag

bsd 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
bsd 
interaction domain residue 545
mutation disrupting interaction
aa 545

Q545 coordinates relative to bsd-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Yang et al., 2022)
FBrf0252671-3.coIP.WB
Reference
Yang et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
bsd 

Bsd

bait

Myc tag

bsd 
polo 

Polo

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
bsd 
interaction domain residue 545
mutation disrupting interaction
aa 545

Q545 coordinates relative to bsd-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Yang et al., 2022)
FBrf0252671-8.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
bsd 

Bsd

enzyme

GST tag

bsd 

Bsd

enzyme

GST tag

polo 

Polo

enzyme target

GST tag

polo 

Polo

enzyme target

GST tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 0 )
References (1)