Moi
GST tag
Peo
FLAG tag
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected HeLa cell extract.
HA tag
Interaction in vivo; bait produced from transfected construct; prey produced from transfected construct.
Two distinct regions of peo (the N-terminal helix, as well as the UEV domain plus C-terminal helices) are sufficient to interact with moi in this assay.