FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rgk1, Ubx
FlyBase ID
FBig0000121647
Interaction Network
Interactions Browser links
Rgk1 network
Ubx network
Reported Interactions
FBrf0252482-12.XL
Reference
Carnesecchi et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rgk1 

Rgk1

bait

Cy3 label

Ubx 

Ubx synonym

prey

MBP tag

Ubx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ubx 
Homeodomain
mutation decreasing interaction
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as a recombinant fusion protein in bacterial system.

(Carnesecchi et al., 2022)
FBrf0252482-3.coIP.PCR
Reference
Carnesecchi et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Ubx 

Ubx

bait

GFP tag

Ubx 
Rgk1 

Rgk1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ubx 
DNA-binding Homeodomain residue 51
mutation decreasing interaction
aa 51

N51A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2R+ cell line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Carnesecchi et al., 2022)

Expression of wildtype Ubx results in enrichment of alternatively spliced exons 11, 13 and 19 of Rgk1. The Ubx N51A mutation significantly decreases enrichment of exon 11, 13 and 19 of Rgk1.

(Carnesecchi et al., 2022)
FBrf0252482-8.XL
Reference
Carnesecchi et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rgk1 

Rgk1

bait

Cy3 label

Ubx 

Ubx synonym

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Ubx binding was weaker for Rgk1 constitutively expressed exon 1 probe compared to alternatively spliced exon 19 probe.

(Carnesecchi et al., 2022)

Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as a recombinant fusion protein in bacterial system.

(Carnesecchi et al., 2022)
FBrf0262829-10.XL
Reference
Blanco et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rgk1 

Rgk1

bait

Cy3 label

Rgk1 
Ubx 

Ubx

prey

MBP tag

Ubx 
Ubx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rgk1 
exon 13
sufficient binding region
unspecified
Ubx 
homeodomain α3 region
necessary binding region
aa 327-345

; coordinates relative to Ubx-PE

Ubx 
homeodomain residue 58
mutation decreasing interaction
aa 58

K58A; within the context of full-length Ubx (aa 1-380); coordinates relative to Ubx-PE

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as a recombinant protein in bacterial system.

(Blanco et al., 2025)
FBrf0262829-4.coIP.PCR
Reference
Blanco et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Ubx 

Ubx

bait

GFP tag

Ubx 
Ubx 
Rgk1 

Rgk1

prey
Rgk1 
Rgk1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rgk1 
exon 13
sufficient binding region
unspecified
Rgk1 
exon 19
sufficient binding region
unspecified
Ubx 
Homeodomain residue 51
mutation disrupting interaction
aa 51

N51A; coordinates relative to Ubx-PE

Ubx 
homeodomain residue 58
mutation decreasing interaction
aa 58

K58A; within the context of full-length Ubx (aa 1-380) or HD (aa 295-380); coordinates relative to Ubx-PE

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Blanco et al., 2025)

Alternatively spliced exon 13 was reverse-transcribed from Rgk1 mRNA. Co-expression of wild-type Ubx with N51A Ubx moderately restores Ubx-Rgk1 interaction. Ubx N51A-K58A mutant fails to associate with RNA exons int he presence of Ubx wild-type.

(Blanco et al., 2025)
External Crossreferences and Linkouts ( 0 )
References (2)